. Author manuscript; available in PMC: 2020 May 1.

Published in final edited form as: Wiley Interdiscip Rev Dev Biol. 2018 Dec 18;8(3):e339. doi: 10.1002/wdev.339

Table 5.

Comparison of the media composition of Schneider’s media (SM), Shield and Sang’s M3 media and ZB media. The formulation of Schneider’s, M3 and ZB media were reproduced based on a comparison of the table compiled by Echalier et al. (2017) with components described by Schneider’s media (Sigma) and Shields and Sang M3 media (Sigma).

	SM			M3			ZB
	mg/L	mM		mg/L	mM		mg/L	mM

Salts
CaCl₂	600	5.40	CaCl₂^. 6H₂O	1500	6.8	CaCl₂	111	1.0
MgSO₄^. 7H₂O	3700	15.00	MgSO₄^. 7H₂O	4400	17.9	MgSO₄	600.6	5.0
KCl	1600	21.50	KHCO₃	500	5.0	KCl	2000	26.8
KH₂PO4	450	3.30	C₅H₈KNO₄^.H₂O	788	38.8
Na₂HPO4	700	4.90				CH₃COONa^.3H₂O	25	0.18
NaHCO₃	400	4.70	Na₂PO₄^.2H₂O	880	5.6	NaH₂PO₄^.H₂O	420	3.0
NaCl	2100	35.90	C₅H₈NaNO₄	653	38.6	NaCl	3200	54.7
Amino acids
L-⍺-Alanine	-	-	L-⍺-Alanine	250	2.8	L-⍺-Alanine	45	0.50
L-β-Alanine	500	5.60	L-β-Alanine	500	2.9	L-β-Alanine	45	0.50
L-Arginine	400	2.30	L-Arginine	300	2.25	L-Arginine	177.3	1.00
						L-Asparagine^.H₂O	171.1	1.14
L-Aspartic acid	400	3.00	L-Aspartic acid	300	2.27	L-Aspartic acid	133	1.00
L-Cysteine HCl	60	0.50	L-Cysteine HCl	200	1.27	L-Cysteine HCl^.H₂O	351	2.00
L-Cystine	100	0.40
						Alanyl-glutamine (Aln-gln)	2600	11.96
L-Glutamic acid	800	5.40				L-Glutamic acid	406.5	2.76
L-Glutamine	1800	12.30	L-Glutamine	600	4.1	L-Glutamine	680	4.64
Glycine	250	3.30	Glycine	500	6.6	Glycine	750	10.00
L-Histidine	400	2.60	L-Histidine	550	3.5	L-Histidine	155	1.00
						L-Hydroxyproline	65	0.50
L-Isoleucine	150	1.10	L-Isoleucine	250	2.0	L-Isoleucine	131	1.00
L-Leucine	150	1.10	L-Leucine	400	3.00	L-Leucine	131	1.00
L-Lysine HCl	1650	9.0	L-Lysine HCl	850	4.60	L-Lysine HCl	182	1.00
L-Methionine	800	5.40	L-Methionine	250	1.60	L-Methionine	149	1.00
						L-Ornithine-HCl	84	0.50
L-Phenylalanine	150	0.90	L-Phenylalanine	250	1.50	L-Phenylalanine	165	1.00
L-Proline	1700	14.8	L-Proline	400	3.50	L-Proline	115	1.00
L-Serine	250	2.40	L-Serine	350	3.30	L-Serine	525	5.00
L-Threonine	350	2.00	L-Threonine	500	4.20	L-Threonine	119	1.00
L-Tryptophan	100	0.50	L-Tryptophan	100	0.50	L-Tryptophan	102	0.50
L-Tyrosine	500	2.70	L-Tyrosine	250	1.40	L-Tyrosine 2Na^. 2H₂O	112.6
L-Valine	300	2.50	L-Valine	400	3.60	L-Valine	117	1.00
Sugars
Glucose	2000	11.00	Glucose	10000	55.50	D-Glucose	2000	11.00
Trehalose	2000	5.50				Trehalose	10000	26.43
Organic acids
⍺-Ketoglutaric acid	200	1.40
Fumaric acid	100	0.80
Malic acid	100	0.75				Malic acid	670	5.00
Succinic acid	100	0.80
			Oxaloacetic acid	250	1.90	Oxaloacetic acid	250	1.90
						Sodium pyruvate	110	1.00
						Succinic acid	60	0.50
Supplements
TC Yeastolate	2000		TC Yeastolate	1000
Peptone	5000
			Bis-Tris (Sigma)	1050	5.0
			Glutathione	0.005
						1-Thioglycerol (β-ME)	5.4	0.05
						Inosine	2.7	0.01
						Thymidine	2.4	0.01
						Uridine	2.4	0.01
						DL-Carnitine-HCl	1000	5.05
						Human insulin	5.0
						Spermidine trihydrochloride		0.001
Vitamins							mg/L	μM
						Vitamin B₁₂ (cobalamin)	0.10	0.07
						Biotin (B₇)	0.10	0.41
						Folic acid (B₉)	0.10	0.23
						Niacinamide (B₃)	0.10	0.82
						D-Calcium panthothenate (B₅)	10.00	40.00
						Pyridoxal hydrochloride (B₆)	30.00	147.33
						Riboflavin (B₂)	0.01	0.03
						Thiamin hydrochloride (B₁)	10.00	29.65
						Nicotinic acid (B₃)	10.00	81.3
						Pyridoxine (B₆)	0.10	0.49
						Choline chloride	20.00	0.14
						m-Inositol	20.0	111.0
						L-⍺-Lecithin (distearoyl)	0.01	0.013
						L-⍺-Lecithin (dimyristoyl)	0.01	0.015
						L-⍺-Lecithin (dipalmityl)	0.005	0.007
						Sodium-⍺-glycerophosphate	0.16	0.74
						Calcium phosphoryl choline	0.13	0.39
						Phosphoethanolamine	0.07	0.50
						Cholestrol	0.002	0.005
						Oleic acid	0.05	0.18
						Linoleic acid	0.05	0.18
						Ferrous sulphate^. 7H₂O	0.95	3.42
						Zinc sulphate^. 7H₂O	0.99	3.44
						Copper Sulphate^. 5H₂O	0.002	0.008
						Manganese Chloride ^. 4H₂O	0.69	3.49
						L-Ascorbic acid 2 -phosphate sesquimagnesium salt hydrate	23.13	78.89