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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Neurochem Res. 2018 Oct 5;44(6):1446–1459. doi: 10.1007/s11064-018-2650-4

Figure 7. Human and mouse hybrid constructs.

Figure 7.

A. Schematic representation of constructs generated to include human and mouse sequences.

B. HEK293FT cells were transfected in triplicate with flag-tagged versions of the indicated hybrid constructs and blotted for LRRK2 (upper panel). Cyclophilin B is used as a loading control for each lane.

C. Quantification of protein expression in (B) related to cyclophilin B. Error bars indicate SEM, n=3. *, p<0.05; **, p<0.01; ****, p<0.0001; ns, non-significant by Tukey’s post-hoc test compared to WT human LRRK2 from one way ANOVA (F6,14=43.8, p<0.0001, n=3 experiments).

D. Quantification of n=3 independent experiments using hybrid constructs of the phosphorylation of the Nictide peptide. ****, p<0.0001; ns, non-significant by Tukey’s post-hoc test compared to WT human LRRK2 from one way ANOVA (F6,14=54.61, p<0.0001, n=3 experiments).