Fig. 1.

The representative record of kinetics of biofilm formation by PAR5. a Density of PAR5 population at the indicated points of time is expressed in CFU of sessile cells per ml. The culture started with the bacterial suspension at a density of 10⁷ CFU/ml. b Building of biofilm matrix. Congo red staining was used for measuring biofilm formation (EPS accumulation). Plot depicts mean values ± SD of absorbance (λ = 492 nm). The mean was calculated on the basis of three independent experiments (n = 3). ^a8-h PAR5 planktonic bacteria cell attachment and the beginning of biofilm formation ^aa72 h—mature biofilm. At these time points of the culture, the concentration of DNA, LPS and EPS in bacterial conditioned media is shown. c SEM of: 0-h PAR (free-floating planktonic bacteria); 8-h PAR5 (cluster of planktonic bacteria, the beginning of biofilm formation); 24-h PAR5 (conglomerate cluster of bacteria—early biofilm)