Fig. 5.

Expression of iNOS, COX-2, and Arg-1 proteins in neutrophils exposed to PAR5 or bacterial-conditioned media. Expression levels of iNOS (a), COX-2 (c), and Arg-1 (e) were measured by western blot analysis of cell lysates collected from 24-h cultures of neutrophils (5 × 10⁵/well). Neutrophils were activated with tested bacteria at a ratio of 20:1 and 100:1 of PAR5:neutrophils, with bacterial-conditioned media (the % of total volume) or with LPS (0.1 µg/ml) as a control. Densitometric analysis of bands from two experiments is shown. Data are normalized to constitutive expression levels of β-actin. In the same experiments, levels of NO₂ (b) and PGE₂ (d) in supernatants were measured. *p < 0.05, **p < 0.005 for 5% BCM-8 h vs. 5% BCN-72 h, one-way ANOVA