Fig. 7.
Effect of LPS and DNA inhibitors on stimulatory properties of BCM-72 h. Neutrophils at 5 × 105/well were cultured with 20% BCM-72 h: (i) untreated, (ii) treated with DNA inhibitors (DNase and/or chloroquine), (iii) treated with inhibitor of LPS (PMx), (iv) treated with all tested inhibitors. TNF-α (a), IL-6 (b) and IL-10 (c) levels were analyzed by ELISA in supernatants collected from 24-h cell cultures. Data are mean ± SEM values of three independent experiments. Each group was run in triplicates. *p < 0.05, **p < 0.005, ***p < 0.001 between treated and untreated BCM-72 h, one-way ANOVA