Fig. 8.
Cytokine production by neutrophils exposed to agents isolated from BCM-72 h. Levels of TNF-α (a), IL-6 (b), IL-12p40 (c), and IL-10 (d) were analyzed by ELISA of supernatants collected from 24-h cultures of neutrophils (5 × 105/well). Neutrophils were stimulated with tested agents isolated from PAR5 BCM at concentrations: LPS—100 ng/ml, EPS—30 µg/ml and DNA—3 µg/ml. In two last groups, the cells were preincubated for 1 h with EPS and then re-stimulated with LPS or DNA. After 24 h, supernatants were collected and the amounts of cytokines were estimated. Data are mean ± SEM values of three independent experiments. Each group was run in triplicates. As a control, LPS from E. coli was used (LPS, 100 ng/ml). *p < 0.05, **p < 0.005, ***p < 0.001 vs. LPS E.coli, one-way ANOVA