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. 2019 Apr 5;9:5742. doi: 10.1038/s41598-019-42008-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mechanical characterisation of metastatic and healthy prostate cell lines. (a) Temporal profiles of the cellular mechanical response estimated as median deviation from the perfect roundness $E_{c o r r e c t e d} = 1 - R_{c o r r e c t e d}$ for DU145 (n = 2199) and PNT2 (n = 2852) cells. The error bars represent the 95% confidence intervals. Data collected over 12 independent experiments. The two profiles were significantly different at all positions (p < 0.0001, Mann-Whitney U test). (b,c) Density scatterplots of the E_corrected (maximum deviation from perfect roundness) versus the initial diameter D_in of each of the DU145 and PNT2 cells analysed in (a). The density of the data points in (b,c) is given by colour, with blue corresponding to the lowest density and yellow to the highest.