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. 2019 Apr 5;10(4):312. doi: 10.1038/s41419-019-1541-1

Fig. 4. SET8 promoted aerobic glycolysis by suppressing SIRT4.

Fig. 4

a Relative mRNA level of SIRT4 in the indicated HCC cell lines with either SET8 overexpression or control vector detected by real-time PCR. b Protein levels of SIRT4, H4K20me1, and SET8 in the indicated HCC cell lines with either SET8 overexpression or control vector detected by western blotting. c ChIP assay of H4K20me1 occupancy at the promoter region of SIRT4. Normal IgG was used a control, and 1% of the total cell lysates was subjected to PCR before immunoprecipitation (input control). d Analysis of the extracellular acidification rate (ECAR) in the indicated HCC cell lines with either SIRT4 overexpression or control vector. e, f The expression levels of genes involved in glucose metabolism in the indicated HCC cell lines with either SIRT4 overexpression or control vector detected by real-time PCR and western blot analysis. Data are shown as mean ± SD of three independent experiments. *P < 0.05