Fig. 6. SET8 regulated SIRT4 expression in a KLF4-dependent manner.

a, b The RNA and protein levels of SIRT4 detected by real-time PCR and western blotting in KLF4-overexpressing cells. c, d Representative IHC images of SIRT4 and KLF4 staining in paired HCC tumor tissues and mice model tumor tissues. Representative images of the immunostainings at ×20 magnification are shown. Scale bar, 50 μm. e KLF4 occupancy of the SIRT4 promoter detected by the ChIP assay. f Cells were transfected with the control vector, SET8 or the dominant negative mutant form SET8 mutant plasmid (SET8^R25G). Extracts were analyzed for western blot analysis. g SET8 decreased the KLF4 occupancy of the SIRT4 promoter, whereas SET8^R25G had little influence. Data are shown as mean ± SD of three independent experiments. ^*P < 0.05