Table 1.

Conditions for the different CYP isoform-specific inhibition assays.

CYP isoform	Microsome concentration [mg/mL]	Specific substrate	Specific inhibitor	Incubation time [min]	Generated metabolite
CYP1A	0.25	0.5 µM ethoxyresorufin	Max. 3 µM alpha-naphthoflavone	5	resorufin
CYP2C9	1	120 µM tolbutamide	Max. 10 µM sulphaphenazole	60	4-hydroxy-tolbutamide
CYP2C19	0.5	25 µM mephenytoin	Max. 50 µM tranylcypromine	60	4-hydroxy-mephenytoin
CYP2D6	0.5	5 µM dextromethorphan	Max. 3 µM quinidine	5	dextrorphan
CYP3A4	0.1	2.5 µM midazolam	Max. 3 µM ketoconazole	5	1-hydroxy-midazolam