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. 2019 Apr 5;10(4):309. doi: 10.1038/s41419-019-1518-0

Fig. 4. DNAJC3 acts as co-transporter for glucose-regulated protein 78 (GRP78) surface translocation.

Fig. 4

a–c GRP78 and DNAJC3 protein expression on the surface and total protein by western blots in INS-1E exposed to human interleukin (hIL)-1β (50 U/ml) and rat interferon (rIFN)-γ (500 U/ml) (16 h; n = 3) (a); EndoC-βH1 exposed to hIL-1β (50 U/ml), hIFN-γ (1000 U/ml), and mouse tumor necrosis factor (mTNF)-α (1000 U/ml) (24 h; n = 3) (b); and MIN6 exposed to hIL-1β (50 U/ml), mIFN-γ (250 U/ml), and mTNF-α (1000 U/ml) (16 h; n = 3) cells (c). d Surface and total GRP78 protein levels in MIN6 transfected with control scramble (10 nM) and DNAJC3 (10 nM) small interfering RNA for 48 h and exposed to cytokines for 16 h (n = 4). Upper panels show one representative western blot out of four independent experiments. Lower panels show the relative intensities of the different protein bands after quantification by densitometry and expressed as a ratio. Data are presented as mean ± SEM and statistically analyzed by two-tailed unpaired Student’s t test. Significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001