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. 2019 Apr 5;9:5682. doi: 10.1038/s41598-019-41952-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Measuring anti-IFN-γ autoAbs by colorimetric ELISA. (a) The protocol of ELISA for measuring anti-IFN-γ autoAbs has been modified from conventional IFN-γ ELISA assay by coating with capture Ab (cAb) and adding recombinant human IFN-γ (rhIFN-γ) and tested serum followed by adding detection Ab (dAb). (b) High production of anti-IFN-γ autoAbs in tested patient sera (P1, P2, and P4). (c) Measuring anti-IFN-γ autoAbs at various dilutions of patient serum as indicated. H1, healthy control 1. Quantitative data (optical density, O.D.) are shown as mean ± SD from three independent experiments. ^*p < 0.05 and ^***p < 0.001, compared with H1.