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. 2019 Apr 5;9:5682. doi: 10.1038/s41598-019-41952-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Measuring anti-IFN-γ autoAbs by immunoblot analysis. (a) The protocol of Western blot (WB)-based analysis for measuring anti-IFN-γ autoAbs has been modified by loading recombinant human IFN-γ (rhIFN-γ) through a polyacrylamide gel for protein separation and then the protein was transferred to a PVDF membrane. After blocking with BSA and incubating with patient serum, detection Ab (dAb) was added. (b) Representative immunoblot showing high binding activity of anti-IFN-γ autoAbs in tested patient sera (P1, P2, and P4). Relative density of measured proteins is also shown. Full-length blots/gels are presented in Supplementary Figure 1.