Figure 6.

R. microsporus can utilise enterobactin as a xenosiderophore. (A) R. microsporus spores were exposed to 50% supernatants harvested from P. aeruginosa PAO1, P. aeruginosa PA14, B. cenocepacia, S. aureus, and E. coli for 24 h. Fungal growth was determined by absorbance (OD₆₀₀) and normalised to control. (n = 6). (B) Concentration of siderophores produced by bacteria was determined by using the Siderotec Assay (EmerginBio). Correlation between total siderophore production and fungal growth was determined by performing a linear regression with Pearson correlation (n = 3). (C) R. microsporus spores were exposed to varying concentrations of purified enterobactin for 24 h at 37 °C (n = 3). Fungal growth was determined by absorbance (OD₆₀₀) and normalised to control. (D) Enterobactin was added to PAO1 sterile supernatants diluted 50% with SAB to a final concentration of 50 μM and incubated at 37 °C for 24 h. Scale bar represents 20 μm. All data was analysed by a Kruskal-Wallis test with Dunn’s multiple comparisons test unless indicated otherwise. *p < 0.05, **p < 0.01, ***p < 0.001.