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. 2019 Apr 5;10(4):313. doi: 10.1038/s41419-019-1543-z

Fig. 8. Nrf2 deficiency strengthened autophagy caused by Lico A in mice with LPS/GalN-induced ALI.

Fig. 8

WT and Nrf2−/− mice were intraperitoneally injected Lico A (100 mg/kg) with mice for twice at a 12-h (interval for 12 h), followed by subjected to LPS/GalN (30 μg/kg; 600 mg/kg). Liver tissues were collected from the mice 6 h after LPS/GalN challenge and analyzed by western blotting. a Effects of Lico A on the protein expression of Atg7, Atg16, Beclin-1, Atg5, Atg12, Atg3, and LC3 conservation were measured by western blot. Moreover, anti-Atg5 and anti-Atg12 antibodies detect the identical bands corresponding to Atg12-Atg5 conjugates. b–h Quantification of relative protein expression was performed by densitometric analysis. β-actin was used as an internal control. Similar results were obtained from three independent experiments. All data are presented as means±SEM (n = 5 in each group). ##p < 0.01 vs. WT Control group; *p < 0.05, **p < 0.01 vs. WT LPS/GalN group; ^^p < 0.01 vs. WT Lico A group; +p < 0.05, ++p < 0.01 vs. WT LPS/GalN+Lico A group; $$p < 0.01 vs. KO Control group; &p < 0.05, &&p < 0.01 vs. KO LPS/GalN group