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. 2019 Apr 5;9:5740. doi: 10.1038/s41598-018-36456-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FGFR3-mediated regulation of ETV5 expression. (a) ETV5 mRNA and (b) ETV5 and FGFR3 protein levels in confluent TERT-NHUC with exogenous overexpression of wildtype (WT) or three types of mutant FGFR3 (S249C, Y375C, and K652E), and in control cells transduced with an empty vector (Control); (c) ETV5 protein levels following FGF1 treatment of TERT-NHUC overexpressing wildtype (WT) FGFR3 and of control cells transduced with the empty vector (Control). ETV5 mRNA was relatively quantified using Taqman Real-Time RT-PCR with SDHA as internal control, while ETV5 and FGFR3 proteins were visualized by western blotting with specific antibodies using alpha-tubulin or beta-actin as loading control. All experiments were repeated in triplicate. ‘*’ indicates a statistical significant difference compared with the control sample.