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. 2019 Apr 5;9:5740. doi: 10.1038/s41598-018-36456-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ETV5 expression and cell density of normal urothelial cells are mutually dependent. (a) Growth curves of TERT-NHUC overexpressing ETV5 and control cells transduced with the empty vector; (b) Growth curves of TERT-NHUC with knockdown of ETV5 (sh155 and sh1189), and control cells transduced with the empty vector (Control) or a scrambled shRNA; (c) mRNA relative expression of ETV5 in uncultured NHUC (passage zero, P0), and cultured mortal NHUC in sub-confluent (NHUC sub) and confluent conditions (NHUC confl). ETV5 mRNA expression levels were relatively quantified using Taqman Real-Time RT-PCR with SDHA as internal control. All experiments were repeated in triplicate. ‘*’ indicates a statistical significant difference between samples.