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. 2019 Apr 5;9:5661. doi: 10.1038/s41598-019-41506-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Trial summary. The findings from this trial are summarised in a colour coded table. Grey represents fully protected vaccinees, orange represents vaccinees eliciting effective T cell and/or humoral immunity, with some showing partial protection, and clear represents unprotected vaccinees with no effective humoral and/or T cell immunity. Yellow boxes represent systemic poly-functionality >30% and green represents mucosal poly-functionality >30%. Animals 9CBC and DA45 were euthanised on days 126 pc and 154 pc respectively, and 9B7A and DD1B on day 210 pc, all prior to the endpoint of the trial and are indicated as “^”. Unprotected animals B68D and CE71 were euthanised on days 126 and 156 respectively, and are indicated as “^^”. The percentage Gag-specific polyfunctionality (†) column represents the percentage of cells expressing combinations of IFN-γ, TNF and IL-2. Pol-specific IL-2 cell counts are presented using an asterisk system (*)where *<0.2; **0.2–0.4; ***0.4–0.6 and ****>0.6.