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. 2019 Apr 5;10(4):307. doi: 10.1038/s41419-019-1547-8

Fig. 4. DBP-mediated AKT activity by regulating PTEN expression in germ cells.

Fig. 4

a qRT-PCR was used to detect the efficiency of PTEN knockdown in GC-1 and GC-2 cells. b qRT-PCR was used to detect PTEN expression after treated with DBP in GC-1 and GC-2 cells. c, d GC-1 (c) and GC-2 (d) cells were transfected with PTEN siRNA or treated with DBP alone, and together, respectively. And then samples were analyzed for PTEN, AKT, p-AKT, PI3K, and p-PI3K expression. All measurements are shown as the means ± SD from three independent experiments, ****p < 0.001