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. 2019 Apr 5;9:5692. doi: 10.1038/s41598-019-42245-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PCC0208027 on cell cycle progression. (a) HCC827 and (b) NCI-H1975 cells were fixed and stained with PI following treatment with PCC0208027, erlotinib, and osimertinib for 24 h. Cell cycle distribution was then detected using flow cytometry. PCC0208027 and osimertinib induced G0/G1 arrest in both HCC827 and NCI-H1975 cells, but erlotinib did not in NCI-H1975 cells. Columns, mean; bars, s.d. (n = 3). ^***P < 0.001, compared to control group. ^###P < 0.001, compared to erlotinib group. (c,d) HCC827 and NCI-H1975 cells were treated with various concentration of PCC0208027, erlotinib, and osimertinib for 24 h. The proteins associated with cell cycle were checked by western blot assay. PCC0208027 treatment significantly decreased the expression of CDK2, CDK4 and the nuclear transcription factor E2F. The full-length blots are presented in the Supplementary Materials. The gels were operated under the same experimental conditions in HCC827 and NCI-H1975 cells, respectively.