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. 2019 Apr 5;9:5725. doi: 10.1038/s41598-019-42056-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Prolonged exposure to leptomycin B (LMB) induces accumulation of tubulin in nuclei. BY-2 and U-2 OS cells expressing GFP-tubulin were treated with 100 nM and 10 nM LMB, respectively, and the amount of tubulin in cytoplasm and nucleus was evaluated. Whereas the amount of tubulin in nuclei of both BY-2 (A) and U-2 OS (D) cells was comparable to mock (methanol, MeOH) - treated cells, prolonged exposure to LMB resulted in significant increase of nuclear tubulin signal (A,D) in BY-2 cells (B,C) and U-2 OS cells (E,F). Statistics: Welch Two Sample t-test; p-value = 0.066 (MeOH in A), p-value < 0.001 (LMB in A); p-value = 0.151 (MeOH in D), p-value < 0.001 (LMB in D). Nucleus (n), nucleolus (nc). Scale bar = 10 μM.