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. 2019 Apr 5;9:5695. doi: 10.1038/s41598-019-42131-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DU 145 cells with a mesenchymal phenotype are considered cancer stem-like cells. (A) Analysis of CD44 and CD24 markers in mesenchymal and epithelial DU 145 cells using flow cytometry. Dot plots show representative results from three independent experiments. (B) Quantitation of results shown in (A). Bars show the mean ± SD of the percentage of CD44⁺CD24⁻ cancer stem-like cells (n = 3). (C) Spheroid formation and growth in anchorage-independent conditions in DU 145 mesenchymal and epithelial cells. (D) Quantitation of results shown in (C). Data are shown as the mean ± SD of the number of spheroids per well. Results are from three independent repetitions. (E) ALDH1 activity in DU 145 mesenchymal and epithelial sublines. Data are presented as the mean ± SD of the percentage of cells with active ALDH1. Results are from three independent repetitions.