Mechanical characterization of Huh7 and HCV replicon cells using AFM. Shown is the topography of Huh7 (A) and HCV replicon cells (B) by contact mode imaging. Sample F-d curves (C) and apparent elastic modulus (D) of Huh7 (black, dashed lines, n = 21) and HCV replicon (red, solid lines, n = 23) cells are shown. Sample F-d curves (E) and apparent modulus of elasticity (F) of Huh7 cells (black, dashed lines, n = 34) and HCV replicon cells (red, solid lines, n = 20) with actin and microtubule depolymerized. ∗∗∗p < 0.001 by one-tailed Student’s t-test. To see this figure in color, go online.