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. 2019 Apr 5;9:5691. doi: 10.1038/s41598-019-42177-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Over-expression of TaJAZ1ΔJas results in up-regulation of TaPR1/2 expression and ROS accumulation in response to Bgt. (a) qRT-PCR analysis showing the expression levels of TaPR1 and TaPR2 in the Bgt-infected bread wheat leaves of KN199 and independent pUbi:TaJAZ1ΔJas transgenic lines. (b) qRT-PCR analysis showing the expression levels of TaPOX2 and TaSOD in the Bgt-infected bread wheat leaves of KN199 and pUbi:TaJAZ1ΔJas transgenic lines. (c) H₂O₂ contents in the bread wheat leaves of KN199 and independent pUbi:TaJAZ1ΔJas transgenic lines treated with Bgt. Samples were collected at 24 hpi. Error bars denote ± SD (n = 3). Asterisks (** and *) above the bars represent significant differences between the control and each treatment at P < 0.01 and P < 0.05, respectively (Student’s t test).