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. 2019 Apr 5;9:5691. doi: 10.1038/s41598-019-42177-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TaJAZ1 directly interacts with TaMYC4. (a) Yeast two-hybrid assay showing that TaJAZ1 physically interacts with TaMYC4. AD-TaJAZ1 and BD-TaMYC4 were co-expressed in yeast AH109 cells. The combinations of AD/BD-TaMYC4, BD/AD-TaJAZ1 and AD/BD were employed as negative controls. SD-L/W represents synthetic dextrose medium lacking Leu and Trp; SD-L/W/H means synthetic dextrose medium lacking Leu, Trp and His. (b) Pull-down assay showing the interaction between TaJAZ1 and TaMYC4. GST-TaJAZ1 protein was pulled down by MBP-TaMYC4 protein, and detected using an anti-GST antibody. MBP and MBP-TaMYC4 were detected using anti-MBP antibody. Arrow indicates specific band. Full-length blot is presented in Supplementary Fig. S3. (c) LCI assay showing the interaction between TaJAZ1 and TaMYC4 in N. benthamiana leaves. The images were collected 48 h after infiltration.