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. 2019 Apr 5;9:5691. doi: 10.1038/s41598-019-42177-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular characterization of TaNINJA and MeJA-induced expression pattern of TaNINJA. (a) Sequence alignment of TaNINJA and AtNINJA proteins. Red frame represents the EAR motifs. Blue frame represents a conserved domain. Amino acid variations among TaNINJA and AtNINJA are marked by gray- and white-shaded background. (b) The box diagrams of the protein structures of TaNINJA and AtNINJA. (c) MeJA-induced expression pattern of TaNINJA. qRT-PCR were performed to determine the relative transcript levels of TaNINJA in the MeJA-treated KN199 leaves at different time points. The levels of TaNINJA were normalized against TaGAPDH. Error bars denote ± SD (n = 3) (**P < 0.01, Student’ s t test).