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. 2019 Apr 5;9:5747. doi: 10.1038/s41598-019-42287-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Role of vimentin in modulating Jurkat cell apoptosis in response to LPS. Jurkat cells were transfected with control siRNA, vimentin-specific siRNA or a plasmid expressing vimentin as described in the Materials and Methods. Cells were then cultured for 6, 18 and 24 h in the presence or absence of LPS (10 µg/mL). Panel A: Representative images of TUNEL staining in the presence of LPS. Panel B: Quantitative comparison of TUNEL positivity in cells with or without LPS exposure. *p < 0.05 compared to control cells without LPS; ^#p < 0.05 compared to control cells with LPS. Data shown are from a single representative assessment; the experiments were repeated at least 3 times with similar results.