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. 2019 Feb 26;116(7):1313–1327. doi: 10.1016/j.bpj.2019.02.016

Figure 2.

Figure 2

Comparison between measured and simulated dependence of the FRET efficiency of a FRET pair (consisting of tetramethylrhodamine as a donor, D, and cyanine 5 as an acceptor, A) using Förster’s formula (dashed blue line) and Eq. 46, with the experimental data points and the Förster distance value (R0 = 65 Å) taken from Deniz et al. (39). Fitting residuals (i.e., the square root of the sum of the squares of fitting errors) were 2.54 for Förster’s formula and 2.16 for Eq. 46 using the same Förster radius (see text).