Table 1.

Comparison of Published VRAC Structures

	Deneka et al. (30)	Kasuya et al. (31)	Kefauver et al. (32)	Kern et al. (33)
Subunit composition	LRRC8A homomer; LRRC8A/C heteromer	LRRC8A homomer	LRRC8A homomer	LRRC8A homomer
Organism	mouse	human	human	mouse
Functionality of LRRC8A homomer	small whole-cell currents with compromised activation by low ionic strength upon expression in LRRC8-deficient cells	single-channel currents in liposomes with reconstituted LRRC8A under asymmetric ion and osmolarity conditions	swelling-induced whole-cell currents upon expression in LRRC8-deficient cells	single-channel currents in excised patch from lipid with reconstituted LRRC8A
Symmetry TMD	C6	C3	C6	C6
Symmetry LRRD	C3	C3 (compact); disordered (relaxed)	C3	disordered
Preparation	digitonin	digitonin	digitonin	nanodisks; digitonin
Different states	no	“compact” versus “relaxed”	no	“constricted” versus “expanded”