Figure 3.
MPS peptides decreased TH1‐, TH2‐, and TH17‐related cytokines and specific‐transcriptional factors as well as C5a expression in the lungs. (a) On Day 38, mice were killed, BAL fluids collected, and lung proteins analysed via elisa. (b) Proteins were extracted from homogenized lungs and subjected to Western blotting to assess T‐bet, GATA‐3, RORγt, FOXP3, and β‐actin expressions. (c) BAL fluids were analysed using a mouse cytokine array to screen 40 cytokines, chemokines, and complement and acute‐phase proteins in the BAL. Pixel density plots of C5/C5a, IL‐1Ra, and TIMP‐1 were analysed using ImageJ. (d) Proteins were extracted from homogenized lungs and subjected to Western blotting to assess C5a and GAPDH expressions. These proteins were quantified using ImageJ. Data are expressed as mean ± SD (n ≧ 5). # P < 0.05, compared to the NC group; *P < 0.05, compared to the OVA group