Table 3.

Comparison of Recovery-Curve-Based and Pixel-Based Estimation for Diffusion and Binding and for Some Different Parameters, Showing Results for Estimation of D

D (m2/s)	kon (s−1)	koff (s−1)	a	$M\left({\hat{D}}^{\left(\text{rc}\right)}\right)$	$S\left({\hat{D}}^{\left(\text{rc}\right)}\right)$	$M\left({\hat{D}}^{\left(\text{px}\right)}\right)$	$S\left({\hat{D}}^{\left(\text{px}\right)}\right)$	ER(D)
5 × 10−12	0.05	0.05	0.01	5.12 × 10−12	8.30 × 10−13	4.99 × 10−12	9.18 × 10−14	0.012
5 × 10−12	1.00	5.00	0.01	5.05 × 10−12	4.12 × 10−13	5.01 × 10−12	1.82 × 10−13	0.192
1 × 10−11	5.00	1.00	0.10	1.48 × 10−11	1.37 × 10−11	1.01 × 10−11	8.85 × 10−13	0.004
5 × 10−11	0.10	0.05	0.01	5.82 × 10−11	2.82 × 10−11	5.01 × 10−11	8.81 × 10−13	0.001
5 × 10−11	0.05	0.10	0.05	7.03 × 10−11	4.38 × 10−11	5.00 × 10−11	1.59 × 10−12	0.001
1 × 10−10	0.05	0.10	0.05	1.49 × 10−10	1.29 × 10−10	1.00 × 10−10	3.23 × 10−12	0.001
1 × 10−10	0.50	5.00	0.10	1.72 × 10−10	1.38 × 10−10	1.01 × 10−10	8.71 × 10−12	0.003
5 × 10−10	0.50	0.05	0.01	6.21 × 10−10	3.37 × 10−10	5.04 × 10−10	2.76 × 10−11	0.006
5 × 10−10	5.00	0.50	0.01	5.26 × 10−10	5.61 × 10−11	5.08 × 10−10	4.02 × 10−11	0.438a
1 × 10−10	0.50	5.00	0.01	1.06 × 10−10	1.33 × 10−11	1.00 × 10−10	3.84 × 10−12	0.070

^aThis number is the “worst case.”