Table 4.

Comparison of Recovery-Curve-Based and Pixel-Based Estimation for Diffusion and Binding and for Some Different Parameters, Showing Results for Estimation of k_on

D (m2/s)	kon (s−1)	koff (s−1)	a	$M\left({\hat{k}}_{\text{on}}^{\left(\text{rc}\right)}\right)$	$S\left({\hat{k}}_{\text{on}}^{\left(\text{rc}\right)}\right)$	$M\left({\hat{k}}_{\text{on}}^{\left(\text{px}\right)}\right)$	$S\left({\hat{k}}_{\text{on}}^{\left(\text{px}\right)}\right)$	ER(kon)
5 × 10−12	0.05	0.05	0.01	0.051	0.011	0.050	0.002	0.042
5 × 10−12	1.00	5.00	0.01	0.921	0.208	1.008	0.118	0.280
1 × 10−11	5.00	1.00	0.10	4.580	1.293	5.088	0.466	0.122
5 × 10−11	0.10	0.05	0.01	0.100	0.009	0.101	0.003	0.085
5 × 10−11	0.05	0.10	0.05	0.059	0.017	0.051	0.005	0.058
1 × 10−10	0.05	0.10	0.05	0.053	0.011	0.051	0.004	0.134
1 × 10−10	0.50	5.00	0.10	0.982	1.136	0.574	0.331	0.076
5 × 10−10	0.50	0.05	0.01	0.524	0.166	0.507	0.039	0.057
5 × 10−10	5.00	0.50	0.01	4.870	0.490	5.098	0.480	0.935a
1 × 10−10	0.50	5.00	0.01	0.588	0.326	0.528	0.171	0.263

^aThis number is the “worst case.”