Table 5.

Comparison of Recovery–Curve-Based and Pixel-Based Estimation for Diffusion and Binding and for Some Different Parameters, Showing Results for Estimation of k_off

D (m2/s)	kon (s−1)	koff (s−1)	a	$M\left({\hat{k}}_{\text{off}}^{\left(\text{rc}\right)}\right)$	$S\left({\hat{k}}_{\text{off}}^{\left(\text{rc}\right)}\right)$	$M\left({\hat{k}}_{\text{off}}^{\left(\text{px}\right)}\right)$	$S\left({\hat{k}}_{\text{off}}^{\left(\text{px}\right)}\right)$	ER(koff)
5 × 10−12	0.05	0.05	0.01	0.052	0.011	0.050	0.001	0.020
5 × 10−12	1.00	5.00	0.01	5.639	2.572	5.387	2.445	0.872a
1 × 10−11	5.00	1.00	0.10	2.203	3.437	1.022	0.103	0.001
5 × 10−11	0.10	0.05	0.01	0.051	0.005	0.050	0.001	0.048
5 × 10−11	0.05	0.10	0.05	0.109	0.018	0.103	0.006	0.112
1 × 10−10	0.05	0.10	0.05	0.108	0.014	0.103	0.005	0.139
1 × 10−10	0.50	5.00	0.10	7.497	11.109	5.885	2.987	0.075
5 × 10−10	0.50	0.05	0.01	0.050	0.004	0.050	0.001	0.103
5 × 10−10	5.00	0.50	0.01	0.487	0.029	0.502	0.006	0.040
1 × 10−10	0.50	5.00	0.01	6.269	5.194	5.249	0.920	0.032

^aThis number is the “worst case.”