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. 2019 Jan 3;47(6):3117–3126. doi: 10.1093/nar/gky1302

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Identification of the binding interface of dsRBD on tRNA. (A) (¹H,¹⁵N)-TROSY of ¹⁵N-labeled tRNA^Lys3 in its free state (black) and in the presence of 1 equivalent of dsRBD (red). (B) left: secondary structure of human tRNA^Lys3 showing the nucleotides that are affected upon dsRBD binding (strongly affected in red: Δδ > 0.15 ppm; moderately affected in orange: Δδ > 0.08 ppm; slightly affected in yellow: Δδ > 0.03 ppm). right: Representation on the surface of human tRNA^Lys3 (pdb 1FIR) of the nucleotides affected upon dsRBD binding. The same color code is used. (C) SAXS characterization of dsRBD, human tRNA^Lys₃ and their reconstituted complex in vitro. Left: Normalized Kratky plot for tRBD (blue), tRNA^Lys₃ (red) and their complex (black). Right: ab initio multiphase reconstruction of tRBD-tRNA^Lys₃ complex SAXS data using a three-phase model by MONSA. The experimental curve is shown in black while in red is the theoretical curve from the MONSA model (χ² = 3.78).