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Discrimination plot for insertion of β-C-Fapy•dGTP. The catalytic efficiency of β-C-Fapy•dGTP insertion opposite dC or dA for wild-type pol β and the mutant where Asp276 was substituted with glycine (D276G) are shown. Each horizontal short bar represents the mean of duplicate independent determinations. Additionally, the value for each determination is plotted (small solid circle) to illustrate the reproducibility of these assays.
