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. 2019 Jan 15;47(6):2932–2945. doi: 10.1093/nar/gky1319

Figure 5.

Figure 5.

Position dependence of R to H substitutions in EF-Tu. Left panel: Error frequencies. Green bars, wild type chromosome-encoded EF-Tu from MRE600; blue bars, chromosome-encoded EF-Tu carrying a C-terminal His-tag (K12 strain); teal bars, plasmid-encoded EF-Tu overexpressed in BL21(DE3). Error bars represent the standard deviation of 3–6 biological replicates. Most of the R→H errors included in the analysis result from reading the 5′-CGT-3′Arg codon except for R45H and R234H substitutions (underlined) that occur on the 5′-CGC-3′ codon. Right panel: The positions of the substitutions are shown in the structure of EF-Tu in the complex with tRNAPhe (PDB file 1OB2).