Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 18;47(6):2966–2980. doi: 10.1093/nar/gkz009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — BRCA1^−/−, BRCA1^−/−53BP1^−/−, CtIP^{S332A/−/−} and nuclease-dead MRE11^D20A/− mutants exhibit increased number of ddC-induced DNA double strand breaks. (A) Representative comet images (upper panel) and OpenComet output images (lower panel) of BRCA1-proficient and -deficient cells following treatment with 4 mM ddC for 2 h. (B) Neutral Comet assay monitoring DSB formation in BRCA1^−/−, BRCA1^−/−53BP1^−/−, CtIP^{S332A/−/−} and nuclease-dead MRE11^D20A/− DT40 cells following ddC treatment for 2 h. The bar graph (upper panel) represents median and SD of comet tails. Out of three repeats; n ≥ 150 comets scored for each data set. The box and whisker plot (lower panel) represents median value of comet tails. Whiskers the 10th and 90th percentiles. *P< 0.01 (Student's t-test).