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. 2019 Jan 15;47(6):3142–3157. doi: 10.1093/nar/gky1308

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effects of structure-based mutations. (A and B) The RNA-binding affinities of MLE dsRBD₁₊₂ for R2H1-wild-type and indicated mutants, determined by FP experiments. K_d values and the corresponding standard errors were determined as described in the ‘Materials and Methods’ section. (C) The histogram of the K_d values shows the RNA-binding affinities of R2H1 for MLE RBD₁₊₂-wild-type and indicated mutants, as determined by FP experiments. (D) RIP assays performed with GFP-tagged MLE_FL-wild-type and indicated mutants. The abundance of roX2 RNA was quantified by qRT-PCR using specific primers for roX2. Relative roX2 enrichment (IP/input) in mutants was normalized to wild-type, while the relative roX2 enrichment (IP/input) in wild-type was set to 1.0. Data are presented as the means ± SD. n represents the number of experiments.