Figure 4.

Loganetin regulated the protein expressions of TLR4/JNK/p38 pathway in rhabdomyolysis‐induced acute kidney injury. Seventy‐two hours after glycerol or saline injection, kidney tissue proteins were extracted from the control group and rhabdomyolysis‐induced acute kidney injury mice with or without Loganetin treatment. The samples were analysed for TLR4, p65, p‐p65, JNK, p‐JNK, p38, p‐p38, p21, c‐Jun, Bax, cleaved caspase 3, and β‐actin using specific antibodies. (a) Representative immunoblot analysis. (b) Immunoblot signals were quantified by densitometry and normalized with β‐actin. The (p‐p65/p65)/β‐actin, (p‐JNK/JNK)/β‐actin, (p‐p38/p38)/β‐actin, p21/β‐actin, C‐Jun/β‐actin, BAX/β‐actin, and cleaved caspase 3/β‐actin ratios are shown. Shown are representative blots and expressed as the means ± SD from series with tissues from n = 10; the bars with different superscripts in each panel were significantly different. *P < 0.05 versus Gly + PBS group