Table 2.

Kinetic constants for AtMAPDA and mutants

Mutant	K M (μM)	k cat (s−1)	k cat/KM (M−1 s−1)	Relative kcat/KM
WT	4.14	0.86	0.21	1.0
H15A	ND	ND	ND	ND
N17A	7.12	1.01	0.14	0.69
H65A	5.51	0.69	0.13	0.61
T97A	8.94	0.62	0.07	0.34
T98A	4.52	0.57	0.13	0.62
K100A	6.89	0.49	0.07	0.35
E220A	ND	ND	ND	ND
D295A	ND	ND	ND	ND
D295N	ND	ND	ND	ND
D296A	ND	ND	ND	ND

ND: not determined.