Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 18;47(6):2996–3012. doi: 10.1093/nar/gkz105

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 3. — The Sas10/Utp3/C1D domain interacts with the N-terminus of Mpp10. (A) HA-tagged Mpp10 (HA-Mpp10) and Sas10 (without a tag) were co-expressed in 293T cells for Co-IP analysis using an anti-HA antibody. HA-Mpp10 and Sas10 were detected with their specific antibodies, respectively. (B and C) Co-IP of the endogenous Mpp10 and Sas10 extracted from the 32hpf-old embryos (B, using the anti-Sas10 polyclonal antibody for Co-IP) and adult liver (C, using the anti-Sas10 and anti-Mpp10 polyclonal antibodies for Co-IP, respectively). Co-IP with IgG serves as a negative control. (D–F) Co-IP analysis of the interaction between Myc-tagged Mpp10 (Myc-m0) with HA-tagged Sas10 (s0) and its different derivatives (s1 to s5) constructed as shown in (D). s5 is deleted of 226–307 amino acids in Sas10. Myc-Mpp10 interacted with Sas10 (s0) and its derivatives containing the Sas10/Utp3/C1D domain s2 to s4 (E, Co-IP with an anti-Myc antibody) but not with s5 without the domain (F, Co-IP with an anti-HA antibody). 4×: 4-fold amount of plasmids for transfection. (G–I) Co-IP analysis of the interaction between HA-tagged Sas10 (HA-s0) with Myc-Mpp10 (m0) and its different derivatives (m1 to m8) constructed as shown in (G). HA-Sas10 interacted strongly with Myc-Mpp10 (m0) and its N-terminal derivatives m1 and m2 (H) but with negligible interaction with m3-m8 (H and I). In (D–G), numbers in each construct define the corresponding positions of amino acids in Sas10 (D) and Mpp10 (G). Predicted α-helix domains in Mpp10 and conserved domains in Sas10 are also outlined.