Skip to main content
. 2019 Feb 18;47(6):2996–3012. doi: 10.1093/nar/gkz105

Figure 8.

Figure 8.

Sas10 determines the nucleolar localization of the Mpp10–Imp3–Imp4 complex. (A) HA-Imp3 and HA-Imp4 were transfected into 293T cells alone or co-transfected with Myc-mpp10 or with Myc-mpp10 and sas10. The cells were subjected to co-immunostaining of HA-tag and Myc-tag, Myc-tag and Fib or HA-tag and Fib 48 h post-transfection. Arrows pointed to co-transfected cells; bar, 10 μm. (B) A model depicts the route of delivery of the Mpp10–Imp3–Imp4 complex into the nucleolus. Once Mpp10 is synthesized it will form the complex with Imp3 and Imp4. Sas10 binds to Mpp10 to protect it from being recognized by cytoplasmic Capn3 and meanwhile to facilitate the translocation of Mpp10 (or the Mpp10–Imp3–Imp4 complex) to the nucleus. Sa10 also binds to the nuclear-localized Mpp10–Imp3–Imp4 complex. After that, Sas10 delivers the Mpp10–Imp3–Imp4 complex to nucleolus for assembling the SSU processome/90S pre-ribosome.