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. 2019 Feb 21;47(6):3244–3256. doi: 10.1093/nar/gkz085

Figure 3.

Figure 3.

Expression and recombination characterization at BioDesignER Safe Sites. (A) Circular map of the BioDesignER chromosome with Safe Sites mapped to corresponding genome position and chromosomal arm (replichore). (B) Genetic architecture of dual fluorescent reporter construct (top) and observed expression of reporters when integrated at each Safe Site on the chromosome (bottom). Replicate measurements of normalized expression levels for each reporter arrayed by chromosomal arm on which construct is integrated. (C) ssDNA recombination rates at each Safe Site for four independent recombineering reactions. X-axis denotes transformed oligo(s) (G- for sfGFP, R- for mKate) or ctrl (water). Bar height corresponds to the mean of two measurements and error bars represent span of data. Stacked barplots for each reaction represent population fractions containing one of three possible modifications (sfGFP off, mKate off, or dual off when both reporters inactivated).