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. 2019 Jan 30;47(6):3028–3044. doi: 10.1093/nar/gkz039

Figure 3.

Figure 3.

AtPolIB exhibits limited terminal transferase activity on double-stranded substrates. (A) Denaturing gel showing the lack of terminal transferase activity on a poly-dT ssDNA template incubated in the presence of individual nucleotides. (B) Denaturing acrylamide gel showing AtPolIB exo- nucleotide incorporation on a ds-DNA substrate in the presence of individual nucleotides. (C) AtPolIB performs MMEJ using DNA templates in trans. Denaturing gel showing MMEJ reactions incubated in the presence of individual nucleotides on pss-DNA(8) substrate. (D) AtPolIB performs limited snap-back replication. Denaturing gel showing reactions incubated in the presence of individual nucleotides on a MH-8 substrate with AtPolIA exo- (lanes 1–6) and AtPolIB exo – (lanes 7–12).