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. 2019 Jan 30;47(6):e32. doi: 10.1093/nar/gkz037

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Functional characterization of GATA6-AS targets: (A) GATA6-AS DBD-Capture-Seq peaks are localized within the promoter of genes predicted to be targets of GATA6-AS by TDF. As examples of non-target regions, promoters of genes not up-regulated during cardiac differentiation are shown. (B) ChOP-PCR showing the in vivo association of GATA6-AS with the target genes identified by GATA6-AS DBD-Capture-Seq. (C) Quantitative reverse-transcription PCR (RT-qPCR) analysis of GATA6-AS and the predicted targets (GATA6 and MEIS1) after an ASO-based knockdown of GATA6-AS. (D) RT-qPCR analysis of mesodermal and cardiac mesodermal genes after the ASO-based knockdown of GATA6-AS. Error bars represent standard deviations for n = 3. The P-values were generated by a two-tailed t-test.