Fig. 1.

RBL-2H3 mast cells immediately after addition of ionomycin (5 μg/ml) (middle panel) and 10 minutes after degranulation. Notice membrane projections (white arrow) and released granules and debris (black arrows). MS analysis was performed on whole cell lysates and supernatant after degranulation. Coomassie gel of degranulated (DG) RBL-2H3 demonstrates Lane 1 conditioned media control, Lane 2 conditioned media after DG, Lane 3 is control cell lysate, Lane 4 is cell lysate after DG. Arrows indicate a 27 kD band and a 43 kD band which Mass spectroscopy analysis identified as rMCP-2 and actin, respectively. Mass spectroscopy analyses were done through the UAB Mass Spectroscopy Core Facility.