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Chymase uptake in isolated rat cardiomyoctes. Human chymase added to adult rat cardiomyoctyes (desmin, green) shows chymase (red) uptake (A) is attenuated by pretreatment with Dyngo-4a (30 μM), an inhibitor for dynamin. Dyngo-4a pretreatment blocks transferrin (red) uptake (B). Dyngo pretreatment attenuates myosin breakdown as shown by western blot (C) with a corresponding decrease in chymase uptake.
