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. 2019 Apr 4;5(4):e01397. doi: 10.1016/j.heliyon.2019.e01397

Fig. 8.

Fig. 8

In situ hybridization of Chymase mRNA in ACF rats. Anti-sense (A) and sense (B) chymase mRNA in rat interstitial cells (oval) and endothelial cells of vessels in cross-section (boxes) in the LV of 4 week ACF animals. Cross-section IHC of ACF LV (C) demonstrates chymase (red) in endothelial cells of blood vessel (arrow head) and interstitial cells and mast cells (arrows) with chymase (red) with desmin (green) and DAPI stain of nuclei (blue). IHC of isolated 12 week ACF cardiomyocytes (D) with intracellular chymase (red) and chymase within endothelial cells (arrow head) identified by von Willebrand factor (green). Chymase mRNA from LV cardiomyocytes of age-matched sham and ACF at 3 and 24 hours (h) and 4 and 12 weeks (E). n = 6 hearts per group.