Table 1.
Inhibitors used in chymotryptic assay.
| Enzyme inhibitor | Final concentration | Supplier | Inhibits |
|---|---|---|---|
| E-64 | 50 μM | Sigma E3132 | Cysteine proteases, trypsin |
| Pepstatin A | 50 μM | Sigma P5318 | Acid proteases (aspartyl peptidases) |
| Bestatin hydrochloride | 50 μM | Sigma B8385 | Metalloprotease inhibitor (selective leucine aminopeptidase, aminopeptidase B and triamino peptidase) |
| 4-Chloromercuribenzoic acid | 50 μM | Sigma C5913 | Inhibits enzymes that require unmodified cysteine residues |
| Lisinopril | 50 μM | Sigma L2777 | Angiotensin converting enzyme |
| Amastatin hydrochloride hydrate | 10 μM | Sigma A1276 | Leucine aminopeptidases |
| Epoxomicin | 1 μM | Sigma E3652 | Proteasome |