Table 3.
Multivariable models for effect of AT1R-Ab or HLA DSA positivity on cytokine levels
| Cytokine | AT1R-Ab+ (n = 92) | P | HLA DSA+ (n = 25) | P |
|---|---|---|---|---|
| Linear modelsa | Coefficient (95% CI) | Coefficient (95% CI) | ||
| TNF-α | 0.38 (0.14–0.63) | 0.002 | −0.01 (−0.42 to 0.39) | 0.883 |
| IFN-γ | 0.71 (0.25–1.18) | 0.002 | 0.35 (−0.38 to 1.08) | 0.360 |
| IL-8 | 1.04 (0.61–1.47) | <0.001 | 0.45 (−0.24 to 1.14) | 0.221 |
| Logistic modelsb | OR (95% CI) | OR (95% CI) | ||
| IL-1β | 3.03 (1.32–6.96) | 0.008 | 0.86 (0.25–2.96) | 0.817 |
| IL-6 | 3.89 (1.34–11.36) | 0.012 | 2.74 (0.55–13.58) | 0.155 |
| IL-17 | 2.56 (0.99–6.62) | 0.049 | 1.26 (0.32–4.89) | 0.714 |
AT1R-Ab, angiotensin II type 1 receptor antibody; CI, confidence interval; HLA DSA, human leukocyte antigen donor-specific antibodies; IFN, interferon; IL, interleukin; LLD, lower limit of detection; OR, odds ratio; TNF, tumor necrosis factor.
Individual linear and logistic regression models were created for each cytokine. Linear models were used when <50% of samples were below the LLD (TNF-α, IFN-γ, IL-8) and logistic models were used otherwise (IL-1β, IL-6, IL-17). Viremia and rejection were assessed on the patient level as any viremia or rejection during the follow-up period. Time posttransplantation was significant for all cytokines except IL-6.
Linear regression models adjusted for patient level random effect, time, age, sex, mean HLA mismatch, living versus deceased donor, viremia, and presence of rejection. Cytokines log transformed for analysis.
Logistic regression models adjusted for patient-level random effect, time, age, sex, mean HLA mismatch, living versus deceased donor, and presence of rejection.